FetalBrain - Gestational week associated analysis
Gloria Li
November 5, 2014
Updated: Wed Dec 17 17:11:25 2014
DMR identification with methyl_diff
- Identify DM CpGs
- methyl_diff one-sided p-value \(\le\) 0.005
- delta fractional methylation \(\ge\) 0.5
- fractional methylation of one sample \(\ge\) 0.75
- methyl_diff one-sided p-value \(\le\) 0.005
- Collapse DM CpGs into DMRs
- adjacent DM CpGs have the same DM status;
- distance between adjacent CpGs (size) \(\le\) 300bp;
- No. of CpGs within each DMR \(\ge\) 3.
- adjacent DM CpGs have the same DM status;
Background
UMR summary and sanity check
- On average, there are 1692 17-week UMRs, 428 intersect between Cortex and GE, and 450 13-week UMRs, 76 intersect. The intersect is highly significant.
- Median DMR length is 321, comparable to breast. It’s similar in all chromosomes in 17-week UMRs, but fluctuate more in 13-week UMRs, probably due to small No. of UMRs identified.
- Median No. of CpGs per DMR is 5, also similar to breast.
| Sample | Total.DMR | Hyper.DMR | Hypo.DMR |
|---|---|---|---|
| Cortex-HuFNSC02_Cortex-HuFNSC04 | 1283 | 179 | 1104 |
| GE-HuFNSC02_GE-HuFNSC04 | 3002 | 722 | 2280 |
UMR asymmetry
- On average, there are , 4.8-fold enrichment in total UMR frequency in 17-week compared to 13-week, 8.17 in Cortex, and 3.97 in GE.
GREAT enrichment for GW-associated UMRs
- GREAT terms are strongly associated with neuron differentiation and brain development.
- 17-week UMRs are highly enriched in glial cell development in Cortex, and neural tube development in GE. Intersect of these two cell types show enrichment in cell fate commitment related to different types of brain cells.
- 13-week UMRs are enriched in neuron fate commitment in Cortex, and regionalization in GE. Intersect of these two cell types show no significant enrichment.
UMR Genomic breakdown
- On average, 62.26% of CpGs in UMRs overlap with genebody, and 23.95% of CpGs in UMRs overlap with promoters, 1.78-fold enriched. 44.87% of CpGs in UMRs overlap with CGIs, 5.97-fold than expected by random.
Proximal UMRs and DE genes
- On average, there are 165 UMRs proximally (TSS +/- 1.5kb) associated with protein-coding genes, 18.23% in 17-week UMRs, and 4.88% in 13-week UMRs, much lower than 17-week UMRs.
- Among proximal UMRs, 14.26% of 17-week proximal UMRs are associated with DE genes, 13.64% 13-week UMRs, similar to 17-week UMRs. On average, 20 DE genes are associated with GW UMRs.
- Among proximal UMR assocaited with DE genes, 79.55% 17-week UMRs change in the same direction as DE genes (hyper to DN), much higher than any Fetal Brain UMRs and close to observed in breast. 50% in 13-week UMRs, much lower than 17-week UMRs.
| proximal.DMRs | unique.genes | DE.DMRs | unique.DE.genes | same.direction | |
|---|---|---|---|---|---|
| Cortex04.UMRs | 7 | 7 | 0 | 0 | 0 |
| Cortex02.UMRs | 215 | 215 | 24 | 19 | 21 |
| GE04.UMRs | 37 | 36 | 6 | 5 | 3 |
| GE02.UMRs | 402 | 402 | 64 | 57 | 49 |
Proximal 13-week UMRs in both Cortex and GE
| id | name | description |
|---|---|---|
| ENSG00000189334 | S100A14 | S100_calcium_binding_protein_A14_[Source:HGNC_Symbol;Acc:18901] |
| ENSG00000237693 | IRGM | immunity-related_GTPase_family,M[Source:HGNC_Symbol;Acc:29597] |
| ENSG00000124564 | SLC17A3 | solute_carrier_family_17_(sodium_phosphate),member3_[Source:HGNC_Symbol;Acc:10931] |
Proximal GE 13-week UMRs with DE genes
| id | name | description | DM | DE |
|---|---|---|---|---|
| ENSG00000134954 | ETS1 | v-ets_erythroblastosis_virus_E26_oncogene_homolog_1_(avian)_[Source:HGNC_Symbol;Acc:3488] | hyper | UP |
| ENSG00000136352 | NKX2-1 | NK2_homeobox_1_[Source:HGNC_Symbol;Acc:11825] | hyper | UP |
| ENSG00000165588 | OTX2 | orthodenticle_homeobox_2_[Source:HGNC_Symbol;Acc:8522] | hyper | UP |
| ENSG00000231421 | hyper | DN | ||
| ENSG00000155760 | FZD7 | frizzled_family_receptor_7_[Source:HGNC_Symbol;Acc:4045] | hyper | DN |
Proximal 17-week UMRs with DE genes in both Cortex and GE
| id | name | description | DM | DE |
|---|---|---|---|---|
| ENSG00000255737 | NA | HCG2014417,isoformCRA_aUncharacterized_proteincDNA_FLJ59598_[Source:UniProtKB/TrEMBL;Acc:B7Z718] | hypo | UP |
| ENSG00000167178 | ISLR2 | immunoglobulin_superfamily_containing_leucine-rich_repeat_2_[Source:HGNC_Symbol;Acc:29286] | hypo | UP |
| ENSG00000161999 | JMJD8 | jumonji_domain_containing_8_[Source:HGNC_Symbol;Acc:14148] | hypo | UP |
| ENSG00000051523 | CYBA | cytochrome_b-245,alphapolypeptide_[Source:HGNC_Symbol;Acc:2577] | hypo | UP |
| ENSG00000169515 | CCDC8 | coiled-coil_domain_containing_8_[Source:HGNC_Symbol;Acc:25367] | hypo | UP |
| ENSG00000205927 | OLIG2 | oligodendrocyte_lineage_transcription_factor_2_[Source:HGNC_Symbol;Acc:9398] | hypo | UP |
| ENSG00000189060 | H1F0 | H1_histone_family,member0_[Source:HGNC_Symbol;Acc:4714] | hypo | UP |
| ENSG00000146904 | EPHA1 | EPH_receptor_A1_[Source:HGNC_Symbol;Acc:3385] | hypo | UP |
| ENSG00000165072 | MAMDC2 | MAM_domain_containing_2_[Source:HGNC_Symbol;Acc:23673] | hypo | UP |
UMRs overlapping with TFBS
| TF | UMR17week_Cortex | UMR13week_Cortex | Ratio_Cortex | UMR17week_GE | UMR13week_GE | Ratio_GE |
|---|---|---|---|---|---|---|
| MAZ | 133 | 5 | 26.6000 | 319 | 12 | 26.5833 |
| GTF2F1 | 28 | 1 | 28.0000 | 103 | 2 | 51.5000 |
| ZBTB7A | 97 | 3 | 32.3333 | 201 | 3 | 67.0000 |
| HMGN3 | 66 | 2 | 33.0000 | 131 | 4 | 32.7500 |
| NRF1 | 34 | 1 | 34.0000 | 64 | 1 | 64.0000 |
| MXI1 | 112 | 3 | 37.3333 | 262 | 7 | 37.4286 |
| E2F4 | 50 | 1 | 50.0000 | 110 | 1 | 110.0000 |
| CCNT2 | 87 | 1 | 87.0000 | 148 | 4 | 37.0000 |
| E2F6 | 140 | 1 | 140.0000 | 263 | 8 | 32.8750 |
UMR enrichment at chromosome ends
DE genes summary
- On average, there are 721 genes differentially expressed between 13- and 17-week individuals, 593 DE between GW13 and GW15 individuals, 757 DE between GW15 and GW17, 751 DE between GW13 and GW17.
- Within each cell type, majority of DE genes are shared among different comparisons (hypergeometric p-value = 0), less but still significant genes overlap between cell types. .
- Majority of the GW-associated DE genes are stage-specific.
| UP | DN | DE | GW | |
|---|---|---|---|---|
| cortex01_cortex03 | 284 | 281 | 565 | 17 vs 15 |
| cortex01_cortex04 | 365 | 165 | 530 | 17 vs 13 |
| cortex02_cortex03 | 398 | 283 | 681 | 17 vs 15 |
| cortex02_cortex04 | 497 | 225 | 722 | 17 vs 13 |
| cortex03_cortex04 | 459 | 183 | 642 | 15 vs 13 |
| GE01_GE03 | 587 | 274 | 861 | 17 vs 15 |
| GE01_GE04 | 645 | 238 | 883 | 17 vs 13 |
| GE02_GE03 | 609 | 312 | 921 | 17 vs 15 |
| GE02_GE04 | 610 | 259 | 869 | 17 vs 13 |
| GE03_GE04 | 313 | 232 | 545 | 15 vs 13 |
Stage-specific DE gene profiles
- GW13-15:UP, GW15-17:UP;
No genes - GW13-15:UP, GW15-17:DN;
id name description ENSG00000168542 COL3A1 collagen,typeIII,alpha1_[Source:HGNC_Symbol;Acc:2201] ENSG00000133636 NTS neurotensin_[Source:HGNC_Symbol;Acc:8038] ENSG00000066032 CTNNA2 catenin_(cadherin-associated_protein),alpha2_[Source:HGNC_Symbol;Acc:2510] ENSG00000135063 FAM189A2 family_with_sequence_similarity_189,memberA2_[Source:HGNC_Symbol;Acc:24820] ENSG00000214401 ENSG00000099984 GSTT2 glutathione_S-transferase_theta_2_[Source:HGNC_Symbol;Acc:4642] ENSG00000238083 LRRC37A2 leucine_rich_repeat_containing_37,memberA2_[Source:HGNC_Symbol;Acc:32404] ENSG00000101255 TRIB3 tribbles_homolog_3_(Drosophila)_[Source:HGNC_Symbol;Acc:16228] ENSG00000160284 C21orf56 chromosome_21_open_reading_frame_56_[Source:HGNC_Symbol;Acc:1298] - GW13-15:DN, GW15-17:UP;
id name description ENSG00000022556 NLRP2 NLR_family,pyrindomain_containing_2_[Source:HGNC_Symbol;Acc:22948] ENSG00000225607 NA Uncharacterized_proteincDNA_FLJ60024,highlysimilar_to_Cytochrome_P450_4F12__[Source:UniProtKB/TrEMBL;Acc:B4DNA9] ENSG00000147813 NAPRT1 nicotinate_phosphoribosyltransferase_domain_containing_1_[Source:HGNC_Symbol;Acc:30450] ENSG00000167654 ATCAY ataxia,cerebellar,Cayman_type_[Source:HGNC_Symbol;Acc:779] - GW13-15:DN, GW15-17:DN;
No genes - GW13-15:UP, GW15-17:not DE;
id name description ENSG00000149054 ZNF215 zinc_finger_protein_215_[Source:HGNC_Symbol;Acc:13007] ENSG00000215845 TSTD1 thiosulfate_sulfurtransferase_(rhodanese)-like_domain_containing_1_[Source:HGNC_Symbol;Acc:35410] ENSG00000100336 APOL4 apolipoprotein_L,4[Source:HGNC_Symbol;Acc:14867] ENSG00000163823 CCR1 chemokine_(C-C_motif)receptor1_[Source:HGNC_Symbol;Acc:1602] ENSG00000169220 RGS14 regulator_of_G-protein_signaling_14_[Source:HGNC_Symbol;Acc:9996] ENSG00000203804 C1orf138 chromosome_1_open_reading_frame_138_[Source:HGNC_Symbol;Acc:32041] ENSG00000204520 MICA MHC_class_I_polypeptide-related_sequence_A_[Source:HGNC_Symbol;Acc:7090] ENSG00000080573 COL5A3 collagen,typeV,alpha3_[Source:HGNC_Symbol;Acc:14864] ENSG00000165379 LRFN5 leucine_rich_repeat_and_fibronectin_type_III_domain_containing_5_[Source:HGNC_Symbol;Acc:20360] ENSG00000213402 PTPRCAP protein_tyrosine_phosphatase,receptortype,C-associatedprotein_[Source:HGNC_Symbol;Acc:9667] ENSG00000139155 SLCO1C1 solute_carrier_organic_anion_transporter_family,member1C1_[Source:HGNC_Symbol;Acc:13819] ENSG00000187773 FAM69C family_with_sequence_similarity_69,memberC_[Source:HGNC_Symbol;Acc:31729] ENSG00000133216 EPHB2 EPH_receptor_B2_[Source:HGNC_Symbol;Acc:3393] ENSG00000119737 GPR75 G_protein-coupled_receptor_75_[Source:HGNC_Symbol;Acc:4526] ENSG00000126790 C14orf149 chromosome_14_open_reading_frame_149_[Source:HGNC_Symbol;Acc:20488] ENSG00000162733 DDR2 discoidin_domain_receptor_tyrosine_kinase_2_[Source:HGNC_Symbol;Acc:2731] ENSG00000108231 LGI1 leucine-rich,gliomainactivated_1_[Source:HGNC_Symbol;Acc:6572] ENSG00000161921 CXCL16 chemokine_(C-X-C_motif)ligand16_[Source:HGNC_Symbol;Acc:16642] ENSG00000119673 ACOT2 acyl-CoA_thioesterase_2_[Source:HGNC_Symbol;Acc:18431] ENSG00000135046 ANXA1 annexin_A1_[Source:HGNC_Symbol;Acc:533] ENSG00000215790 SLC35E2 solute_carrier_family_35,memberE2_[Source:HGNC_Symbol;Acc:20863] ENSG00000157150 TIMP4 TIMP_metallopeptidase_inhibitor_4_[Source:HGNC_Symbol;Acc:11823] ENSG00000170579 DLGAP1 discs,large(Drosophila)homolog-associatedprotein_1_[Source:HGNC_Symbol;Acc:2905] ENSG00000143252 SDHC succinate_dehydrogenase_complex,subunitC,integralmembrane_protein,15kDa[Source:HGNC_Symbol;Acc:10682] ENSG00000107796 ACTA2 actin,alpha2,smoothmuscle,aorta[Source:HGNC_Symbol;Acc:130] ENSG00000124193 SRSF6 serine/arginine-rich_splicing_factor_6_[Source:HGNC_Symbol;Acc:10788] ENSG00000152661 GJA1 gap_junction_protein,alpha1,43kDa[Source:HGNC_Symbol;Acc:4274] ENSG00000184110 EIF3C eukaryotic_translation_initiation_factor_3,subunitC_[Source:HGNC_Symbol;Acc:3279] - GW13-15:DN, GW15-17:not DE;
id name description ENSG00000166923 GREM1 gremlin_1_[Source:HGNC_Symbol;Acc:2001] ENSG00000164123 C4orf45 chromosome_4_open_reading_frame_45_[Source:HGNC_Symbol;Acc:26342] ENSG00000008196 TFAP2B transcription_factor_AP-2_beta_(activating_enhancer_binding_protein_2_beta)_[Source:HGNC_Symbol;Acc:11743] ENSG00000186897 C1QL4 complement_component_1,qsubcomponent-like_4_[Source:HGNC_Symbol;Acc:31416] ENSG00000054598 FOXC1 forkhead_box_C1_[Source:HGNC_Symbol;Acc:3800] ENSG00000104808 DHDH dihydrodiol_dehydrogenase_(dimeric)_[Source:HGNC_Symbol;Acc:17887] ENSG00000124813 RUNX2 runt-related_transcription_factor_2_[Source:HGNC_Symbol;Acc:10472] ENSG00000173068 BNC2 basonuclin_2_[Source:HGNC_Symbol;Acc:30988] ENSG00000165929 TC2N tandem_C2_domains,nuclear[Source:HGNC_Symbol;Acc:19859] ENSG00000157064 NMNAT2 nicotinamide_nucleotide_adenylyltransferase_2_[Source:HGNC_Symbol;Acc:16789] ENSG00000243244 STON1 stonin_1_[Source:HGNC_Symbol;Acc:17003] ENSG00000173809 TDRD12 tudor_domain_containing_12_[Source:HGNC_Symbol;Acc:25044] ENSG00000135333 EPHA7 EPH_receptor_A7_[Source:HGNC_Symbol;Acc:3390] ENSG00000231421 ENSG00000168675 C18orf1 chromosome_18_open_reading_frame_1_[Source:HGNC_Symbol;Acc:1224] ENSG00000156395 SORCS3 sortilin-related_VPS10_domain_containing_receptor_3_[Source:HGNC_Symbol;Acc:16699] ENSG00000204103 MAFB v-maf_musculoaponeurotic_fibrosarcoma_oncogene_homolog_B_(avian)_[Source:HGNC_Symbol;Acc:6408] ENSG00000168447 SCNN1B sodium_channel,nonvoltage-gated1,beta[Source:HGNC_Symbol;Acc:10600] ENSG00000106546 AHR aryl_hydrocarbon_receptor_[Source:HGNC_Symbol;Acc:348] ENSG00000117598 NA Lipid_phosphate_phosphatase-related_protein_type_5_[Source:UniProtKB/Swiss-Prot;Acc:Q32ZL2] ENSG00000120322 PCDHB8 protocadherin_beta_8_[Source:HGNC_Symbol;Acc:8693] ENSG00000250305 NA Putative_methyltransferase_KIAA1456_[Source:UniProtKB/Swiss-Prot;Acc:Q9P272] ENSG00000106868 SUSD1 sushi_domain_containing_1_[Source:HGNC_Symbol;Acc:25413] ENSG00000183943 PRKX protein_kinase,X-linked[Source:HGNC_Symbol;Acc:9441] ENSG00000204060 FOXO6 forkhead_box_O6_[Source:HGNC_Symbol;Acc:24814] ENSG00000170941 NA Uncharacterized_protein_KIAA1456_[Source:UniProtKB/Swiss-Prot;Acc:Q8N9K7] ENSG00000063438 AHRR aryl-hydrocarbon_receptor_repressor_[Source:HGNC_Symbol;Acc:346] ENSG00000107902 LHPP phospholysine_phosphohistidine_inorganic_pyrophosphate_phosphatase_[Source:HGNC_Symbol;Acc:30042] ENSG00000169436 COL22A1 collagen,typeXXII,alpha1_[Source:HGNC_Symbol;Acc:22989] ENSG00000184985 SORCS2 sortilin-related_VPS10_domain_containing_receptor_2_[Source:HGNC_Symbol;Acc:16698] ENSG00000163328 GPR155 G_protein-coupled_receptor_155_[Source:HGNC_Symbol;Acc:22951] ENSG00000173598 NUDT4 nudix_(nucleoside_diphosphate_linked_moiety_X)-type_motif_4_[Source:HGNC_Symbol;Acc:8051] ENSG00000114200 BCHE butyrylcholinesterase_[Source:HGNC_Symbol;Acc:983] ENSG00000183722 LHFP lipoma_HMGIC_fusion_partner_[Source:HGNC_Symbol;Acc:6586] ENSG00000139722 VPS37B vacuolar_protein_sorting_37_homolog_B_(S.cerevisiae)[Source:HGNC_Symbol;Acc:25754] - GW13-15:not DE, GW15-17:UP;
id name description ENSG00000145247 OCIAD2 OCIA_domain_containing_2_[Source:HGNC_Symbol;Acc:28685] ENSG00000070748 CHAT choline_O-acetyltransferase_[Source:HGNC_Symbol;Acc:1912] ENSG00000165659 DACH1 dachshund_homolog_1_(Drosophila)_[Source:HGNC_Symbol;Acc:2663] ENSG00000165983 PTER phosphotriesterase_related_[Source:HGNC_Symbol;Acc:9590] ENSG00000139220 PPFIA2 protein_tyrosine_phosphatase,receptortype,fpolypeptide_(PTPRF),interactingprotein_(liprin),alpha2_[Source:HGNC_Symbol;Acc:9246] ENSG00000147255 IGSF1 immunoglobulin_superfamily,member1_[Source:HGNC_Symbol;Acc:5948] ENSG00000146233 CYP39A1 cytochrome_P450,family39,subfamilyA,polypeptide1_[Source:HGNC_Symbol;Acc:17449] ENSG00000181649 PHLDA2 pleckstrin_homology-like_domain,familyA,member2_[Source:HGNC_Symbol;Acc:12385] ENSG00000204516 MICB MHC_class_I_polypeptide-related_sequence_B_[Source:HGNC_Symbol;Acc:7091] ENSG00000164692 COL1A2 collagen,typeI,alpha2_[Source:HGNC_Symbol;Acc:2198] ENSG00000114805 PLCH1 phospholipase_C,eta1_[Source:HGNC_Symbol;Acc:29185] ENSG00000051523 CYBA cytochrome_b-245,alphapolypeptide_[Source:HGNC_Symbol;Acc:2577] ENSG00000145703 IQGAP2 IQ_motif_containing_GTPase_activating_protein_2_[Source:HGNC_Symbol;Acc:6111] ENSG00000165072 MAMDC2 MAM_domain_containing_2_[Source:HGNC_Symbol;Acc:23673] ENSG00000130558 OLFM1 olfactomedin_1_[Source:HGNC_Symbol;Acc:17187] ENSG00000120318 ARAP3 ArfGAP_with_RhoGAP_domain,ankyrinrepeat_and_PH_domain_3_[Source:HGNC_Symbol;Acc:24097] ENSG00000198750 GATSL2 GATS_protein-like_2_[Source:HGNC_Symbol;Acc:37073] ENSG00000162849 KIF26B kinesin_family_member_26B_[Source:HGNC_Symbol;Acc:25484] ENSG00000134333 LDHA lactate_dehydrogenase_A_[Source:HGNC_Symbol;Acc:6535] ENSG00000064666 CNN2 calponin_2_[Source:HGNC_Symbol;Acc:2156] - GW13-15:not DE, GW15-17:DN;
id name description ENSG00000259210 ENSG00000198650 TAT tyrosine_aminotransferase_[Source:HGNC_Symbol;Acc:11573] ENSG00000163053 SLC16A14 solute_carrier_family_16,member14_(monocarboxylic_acid_transporter_14)_[Source:HGNC_Symbol;Acc:26417] ENSG00000125637 PSD4 pleckstrin_and_Sec7_domain_containing_4_[Source:HGNC_Symbol;Acc:19096] ENSG00000153822 KCNJ16 potassium_inwardly-rectifying_channel,subfamilyJ,member16_[Source:HGNC_Symbol;Acc:6262] ENSG00000188803 SHISA6 shisa_homolog_6_(Xenopus_laevis)_[Source:HGNC_Symbol;Acc:34491] ENSG00000127952 STYXL1 serine/threonine/tyrosine_interacting-like_1_[Source:HGNC_Symbol;Acc:18165] ENSG00000150907 FOXO1 forkhead_box_O1_[Source:HGNC_Symbol;Acc:3819] ENSG00000076344 RGS11 regulator_of_G-protein_signaling_11_[Source:HGNC_Symbol;Acc:9993] ENSG00000174938 SEZ6L2 seizure_related_6_homolog_(mouse)-like_2_[Source:HGNC_Symbol;Acc:30844] ENSG00000122694 GLIPR2 GLI_pathogenesis-related_2_[Source:HGNC_Symbol;Acc:18007] ENSG00000167774 NDUFA7 NADH_dehydrogenase_(ubiquinone)1alpha_subcomplex,7,14.5kDa_[Source:HGNC_Symbol;Acc:7691] ENSG00000132164 SLC6A11 solute_carrier_family_6_(neurotransmitter_transporter,GABA),member_11_[Source:HGNC_Symbol;Acc:11044]
DAVID enrichment for DE genes
- GW associated DE genes shared by cell types show no enrichment due to small gene lists.
- GW15 vs GW 13: calcium ion binding in cortex UP and GE DN, neurogenesis in cortex DN.
- GW17 vs GW 15: neurogenesis in GE, no enrichment in cortex.
- GW17 vs GW 13: calcium ion binding in cortex UP and GE UP, neurogenesis in GE DN.
Isoform analysis
- On average, there are NA isoform genes between 13- and 17-week individuals, majority of them are shared among different comparisons and between the two cell types (hypergeometric p-value = 0).
- There are 2878 isoform genes in cortex supported by at least two comparisons, and 2505 in GE, among them, 1800 isoform genes are shared between the two cell types.
- Isoform genes are enriched in cell signaling related terms.
| Sample | DE_genes | DE_exons | with_expressed_genes | isoform_exons | exclude_DE_genes | isoform_genes | GW |
|---|---|---|---|---|---|---|---|
| Cortex-HuFNSC01_Cortex-HuFNSC03 | 565 | 28637 | 16045 | 8044 | 7772 | 2490 | 17 vs 15 |
| Cortex-HuFNSC01_Cortex-HuFNSC04 | 530 | 27428 | 13200 | 6365 | 6134 | 2167 | 17 vs 13 |
| Cortex-HuFNSC02_Cortex-HuFNSC03 | 681 | 28963 | 16765 | 7961 | 7630 | 2425 | 17 vs 15 |
| Cortex-HuFNSC02_Cortex-HuFNSC04 | 722 | 29416 | 13894 | 6351 | 6011 | 2105 | 17 vs 13 |
| Cortex-HuFNSC03_Cortex-HuFNSC04 | 642 | 26826 | 12185 | 5818 | 5479 | 1994 | 15 vs 13 |
| GE-HuFNSC01_GE-HuFNSC03 | 861 | 31361 | 14725 | 5880 | 5458 | 2041 | 17 vs 15 |
| GE-HuFNSC01_GE-HuFNSC04 | 883 | 34359 | 15889 | 6401 | 5996 | 2049 | 17 vs 13 |
| GE-HuFNSC02_GE-HuFNSC03 | 921 | 32827 | 15557 | 5773 | 5306 | 1965 | 17 vs 15 |
| GE-HuFNSC02_GE-HuFNSC04 | 869 | 34699 | 16012 | 6113 | 5711 | 1986 | 17 vs 13 |
| GE-HuFNSC03_GE-HuFNSC04 | 545 | 24752 | 12223 | 4582 | 4422 | 1454 | 15 vs 13 |
